Monochromatic green light stimulation during incubation shortened the hatching time via pineal function in White Leghorn eggs

Abstract Background Effect of monochromatic green light illumination on embryo development has been reported in chickens. The avian pineal gland is an important photo-endocrine organ formed by a mediodorsal protrusion during embryonic development. However, the involvement transduction process remains to be elucidated. In present study, we investigated influence hatching time and explored possible mechanism via function. Results A total 600 eggs White Leghorn (Shaver strain) were incubated under photoperiods either 12 h darkness using (12L:12D group) or 24 (0L:24D for 18 d. Compared 0L:24D group, stimulation shortened without extending hatch window impairing hatchability. liver embryos 12L:12D condition was heavier than those group d 21 post incubation which may linked observed increase serum concentration insulin-like growth factor 1 (IGF-1); primary secretion liver. Histological structure analysis demonstrated that increased follicle area, wall thickness lumen area 10 incubation. Rhythmic function three clock related genes (brain muscle ARNT-like-1, BMAL1 ; circadian locomotor output cycles kaput, CLOCK cryptochrome-1, CRY1 ) melatonin rate-limiting enzyme gene (arylalkylamine N-acetyltransferase, AANAT rhythmically expressed but not group. Simultaneously, also (MT), hepatocyte proliferation IGF-1 previous studies. Conclusions performance. Pineal gland’s early histological maturation its rhythmic accelerated stimulation. It key axis regulates development, potential could through enhanced MT promotes IGF-1.